Data-enriched characterization of mobile populations. Morphological information and facts from visuals can insert into the richness of flow cytometry data. For example, the figure shows an otherwise conventional apoptosis assay making use of Annexin V and PI, introducing cell imaging to characterize cells in Every population to expose morphologically distinct capabilities. These insights couldn't are actually obtained from multiplex staining by yourself.
As samples are acquired on the Attune CytPix Flow Cytometer, the higher-pace brightfield digital camera captures and merchants images of detected gatherings at a level around six,000 photographs per 2nd, with regards to the flow rate and graphic measurement. For higher adaptability, Attune Cytometric Software program permits you to modify the picture seize frequency as essential and select a certain gate for image capture, if essential.
Incorporate acoustic concentrating know-how with higher-parameter Investigation for versatile spectral unmixing and traditional workflows.
The award-profitable Invitrogen Attune CytPix Flow Cytometer combines acoustic focusing using a higher-pace brightfield digital camera for simultaneous substantial throughput flow cytometry and superior resolution brightfield imaging.
"A picture is just not fairly truly worth 1000 dots, however it actually really helps to see pictures. It is great to determine illustrations or photos that correlate with staining. Such as, AnnexinV-beneficial cells which have been morphologically distinct than wholesome cells, or CD14+ monocytes which can be larger and more textured than CD3+/CD4+/CD14- lymphocytes.
Below, an experienced person has gated singlets confidently. Immediately after assessing the handbook singlet gate, the CytPix graphic-derived parameter ParticleCount reveals this gate incorporates in excess of four% aggregates.
Pair your Attune flow cytometer with the autosampler for amplified workflow performance, and incorporate robotic integration for max throughput.
Steady image good quality even at significant throughput Acoustic focusing and also a large-speed digital camera Incorporate to graphic these T cells consistently at minimal or large flow premiums. Effortlessly change target and camera settings to satisfy experimental prerequisites.
Early signs of this contamination had at first been dismissed as particles. Backgating to discover new populations. Backgating imaged cells around the Attune CytPix Flow Cytometer also allows you to use morphological functions to find out interesting subpopulations that might not be obvious from flow cytometry data by itself.
End users can watch party visuals in actual-time to substantiate sample high-quality, optimize protocols, and history the morphology of each mobile populace identified for Examination. Imaging capture also Gains from acoustic concentrating, which really helps to position the cells to ensure that a sharp, centered impression is attained.
AI-driven automated picture Evaluation With all the Attune Cytometric Program interprets function characteristics into unique morphology parameters that can be coupled with regular fluorescence and scatter parameters to expose hugely reproducible inhabitants modifications and exclusive subsets.
Application Observe: Automated impression Examination cuts down consumer-to-person variability in flow cytometry gating strategies
Constructed-in dimensionality reduction applications increased resolution into populations. Fluorescently gated populations back again-gated on to UMAP plot to confirm expected population resolution in lysed entire human blood. Consumer can perspective situations from Each and every cluster for Visible affirmation of mobile phenotypes.
No clean/no lyse leukocyte analysis with imaging. Cells have been acquired from 24-hour-aged blood by dilution in 1 mM get more info EDTA ( Professional UX/UI permits end users to teach AI models for expanded programs. (A) The “Coach Product” characteristic supplies annotation applications and real-time model training precision.
At the guts of each Attune Flow Cytometer lies our groundbreaking acoustic concentrating technologies, which utilizes sound waves to meticulously align cells inside the flow mobile. This ground breaking technique surpasses traditional hydrodynamic approaches, resulting in quicker processing speeds, Fantastic knowledge high-quality, and greater sample throughput.
The Attune Xenith cytometer is usually a substantial-parameter spectral analyzer, supplying each spectral unmixing and traditional flow capabilities for extensive and multipurpose mobile Investigation.
The Attune CytPix Flow Cytometer enables end users to visualize samples during acquisition, confirm the precision of gating strategies, and guidance benefits with trusted documentation for publication reasons.
What seemed to be a homogeneous populace is in fact extra varied—an Perception that needs to be deemed when interpreting outcomes.